Glowell Luminescent Standards

Main Features

The Glowell GLO-014 low light imaging standard (LLIS) emits light over 5 orders of magnitude. It can be used for any low light-measuring camera - for quality control or as a benchmark for between experiment comparisons. This model emits Green light with spectral peak of 525 nm. The Tritium based self-illuminating standards are used to calibrate scientific bioimaging systems (low light cameras). The use of the Tritium standards further allows direct comparison between multiple images.

• For low-light calibration
• Extremely stable emission
• Peak emission at 525nm
• Descending light output of 50%, 10%, 1%, 0.1%, 0.01%
• Calibrated for quantification

Each unit contains a sealed core with a gaseous tritium light source. The level of radioactivity is 900MBq (24.3 mCi) per unit, which conforms to international standards.

Results

Estimating the light output from a biological sample.
Figure 1
Determining the sensitivity of a CCD camera.
Figure 2
Figure 3

Application Notes

Applications notes are listed below for various Glowell standards including the GLO-014.

Use of multiple Glowells™ for assessment of luminometer performance: Part 1- Diagonal bias. Application note G1001

Use of multiple Glowells™ for assessment of luminometer performance: Part 2- Drift of readings over time. Application note G1002

Quantification of light output in a luminescence assay using Glowell™ standards. Application note G1003

Measuring the light output of a bioluminescent fungus with a CCD camera using a Glowell™ Low Light Imaging Standard. Note includes discusses use of the GLO-014. Application note G1004

Using the Glowell™ Low Light Imaging Standard to evaluate dynamic range and sensitivity of a CCD camera. Note includes discusses use of the GLO-014. Application note G1005

Quality control of a bioluminescent assay and standardisation of data using Glowell™ Microplate standards. Application note G1007

Use of multiple Glowells™ for assessment of luminometer performance: Part 3- Characterisation of different luminometers. Application note G1008

Use of Glowells™ to demonstrate effect of luminometer gain settings. Application note G1009

Use of Glowells™ to determine the extent of within well reflection and between well cross talk in a number of different microplates. Application note G1010

Using the Glowell™ Low Light Imaging Standard to evaluate effect of pixel binning settings on effective sensitivity of a CCD camera. Application note G1011

Use of Glowells™ to standardise data across different luminometer models and manufacturers. Application note G1012

Use of Glowells™ for photon-counting luminometer control and comparison across instruments and sites. Application note G1013

Technical Notes:

Rate of decay of Glowell™ light output over time. Technical note T1001

Glowell is a trademark of Lux Biotechnolgoy Ltd.

Ordering Information
Product	Part Number
Glowell GLO-014 LLIS	33-0017-01
GLO15 Blue	33-0018-01
GLO18 Red	33-0019-01

Other Glowell LLIS luminescent standards and 96 Well microplate standards are available. Contact UVP for information.