Application notes








BioImaging Systems Application Notes


UVP's products can be used in many different applications. Application Bulletins describe specific uses for some of UVP's products. For applications not shown here, contact UVP's offices.


Nucleic Acid Gel Documentation

Safe Gel Imaging with Blue Light Excitation Using GelGreen(tm) Dye  FP-170


Quantitation of Lanes and Bands using 1D Analysis  FP-160


Next Generation Gel Imaging Using GelRed™ and GelGreen™ Dyes with the GelDoc-It® Imaging 
System  FP-153


DNA Quantitation Using Agarose Electrophoresis Densitometry  FP-123


Using the UVP BioDoc-It2 Imaging System for Analysis of Differential Display RT-PCR Gels  FP-119


Products Used: GelDoc-It Imaging System and LabWorks Software


 
Protein Gel Imaging

Ultra Sensitive Protein Detection and Imaging with LumiteinT Protein Gel Stain and GelDoc-It Imaging 
System  FP-154


Quantitative DNA and Protein Analysis with a Highly Uniform UV Illumination Imaging System
and Fluorescent Calibration Step Tablet  FP-127


Cytokine Antibody Array Applications with UVP BioImaging System   FP-125


Applications of a Highly Uniform UV Illumination Imaging System for Quantitative DNA and Protein Analysis   FP-124


 
Chemiluminescent Western Blotting

Digital Chemiluminescent Imaging of Protein Blots FP-159


Capture ECLs, Westerns, Chemi Blots with the BioSpectrumAC Imaging System FP-128


Western Blot Imaging with the UVP ChemStudio PLUS (formerly BioSpectrum)


Optimizing Western Blots for Chemiluminescent Detection with Cooled CCD Camera Imaging Systems  FP-111


Chemiluminescent Immunoblot Detection with Enhanced Luminol HRP Substrates and Digital CCD Camera   FP-117


Enhancement of Chemiluminescent Images   FP-112


Acquiring and Calibrating Chemiluminescent Blots with Visible Light Markers  FP-118


 
Fluorescent Western Blotting/Multiplexing

Multiplex Fluorescent Western Blot Detection Using the UVP ChemStudio Plus (formerly BioSpectrum) FP-176


Multiplex NIR Imaging of Protein Blots with the UVP ChemStudio PLUS and eLITE™ MultiSpectral 
Source FP-158


 
Colony Counting

Colony Measurement Tools Using the ColonyDoc-It™ Imaging Station FP-164


Counting GFP Inhibition Zones with the ColonyDoc-It Imaging Station FP-148


Multi-Color Bacterial Counting with the ColonyDoc-It Imaging Station FP-149


Measuring Inhibition Zones with the ColonyDoc-It Imaging Station FP-143


Radial Diffusion Assay and Analysis for Proteases and Other Hydrolytic Enzymes Using the 
ColonyDoc-It Imaging Station FP-147


Colony Counting GFP Bacteria with the ColonyDoc-It Imaging Station FP-142


 
Microspheres

Imaging of Fluorescent Microspheres Using the iBox® Explorer™ Imaging Microscope  FP-175



2D Gel & Blot Imaging

2D Imaging with the UVP ChemStudio PLUS (formerly Biospectrum)  FP-131 2D


 
Small Animal In Vivo Imaging

Infection of Soybean Plant Roots With GFP-Expressing Phytophthora Sojae, Imaged Using the iBox Explorer2 Imaging Microscope FP-180


In Vivo Fluorescence Imaging of Angiogenic Vessels in Liver Cancer Animal Model Using the iBox Explorer2 Imaging Microscope FP-181


Imaging of Tumor Growth and Metastasis in a Pancreatic Cancer Mouse Model FP-186


In Vivo Imaging of a Cy5.5 Labeled Amyloid-β, an Alzheimer's Disease Associated Protein FP-185


Low Light Imaging of Reactive Oxidative Species within Wound Sites Using Luminol  FP-179


Biodistribution Monitoring Using the iBox Explorer2 Imaging Microscope  FP-178


Imaging of Near Infrared (NIR) Dyes In Vivo  FP-173


Monitoring Tumor Progression Using the iBox® Scientia™ Imaging System  FP-172


Intravital Imaging of a Mouse Ventral Skin-Flap  FP-171


Detecting a Mouse Pancreatic Cancer Cell Line Using the iBox® Explorer™ Imaging Microscope  FP-169


Real-Time Imaging of Nuclear-Cytoplasmic Dynamics in UV Light Killing of Cancer Cells Expressing 
Fluorescent Proteins  FP-156


Color Coded Fluorescence Imaging in Mice Using the iBox Small Animal Imaging System  FP-155


Measuring Molecular Mobility with Macro Fluorescence Imaging  FP-151


The Advantages of Fluorescent Proteins Over Luciferase for In Vivo Imaging  FP-129


Salmonella Typhimurium Treatment of RFP Tagged U87 Tumors in Nude Mice (AntiCancer, Inc.)  FP-130


 
Plant Imaging

Use of Red Firefly Luciferase to Monitor Infiltration of Nicotiana benthamiana for In Planta Bioluminescence
FP-184


Infection of Soybean Plant Roots With GFP-Expressing Phytophthora Sojae  FP-180


Genetic Mapping of Newly Identified Mutations Using the Arabidopsis Genome and CAPs Markers  FP-120


 
General Imaging Topics

Using Flat Field Correction to Compensate for Shadowing Effect When Imaging Microtiter Plates FP-122


Understanding and Detection of Green Fluorescent Proteins (GFP) and Their Variants  FP-121


Understanding CCD Cameras  FP-114

Filter Selection Chart 




©2018 UVP Application Bulletins and information can be copied for your own use, but cannot be reprinted or published without permission from Analytik Jena / UVP, LLC.