iBox® Spectra™ In Vivo Imaging System

iBox® Spectra™ In Vivo Imaging System

Fluorescence in vivo small animal imaging research just got easier! Designed for quick screening and image capture, the iBox Spectra includes a high resolution color camera, a touch screen and a high intensity excitation light source to provide optimal conditions for small animal study. The high resolution camera immediately and clearly identifies the area of interest within the animal. Touch screen technology provides a simple interface for capturing images. The UVP eLITE MultiSpectral Light Source provides excitation of florescent proteins.

Applications

 

• umor studies

• Cancer research

• Heart disease

• Gene therapy

System NamesCamera
iBox Spectra Imaging SystemMultiCam 310C

Main Features


  • Completely networkable, stand-alone system for fluorescence in vivo imaging

  • Color CCD camera for high resolution, quick image capture of fluorescence in vivo images

  • Darkroom with integrated touch screen technology and easy to use software interface for live preview, capture and saving images

  • UVP eLITE MultiSpectral Light Source for directed, filtered illumination

  • Roll-out tray inside the darkroom provides easy access for placement of mice on the warming plate

  • VisionWorks Software  


Capture Software

  • Controls via touch screen interface

  • Controls live preview, capture and save functions

  • Select exposure, aperture, histogram, zoon and focus settings as needed

  • Exposure time and other settings display on the slider bar

  • Choose lighting, filters and other functions from the touch screen


Darkroom Capabilities

  • Light tight darkroom creates optimum imaging conditions

  • Unique viewing window for quick sample inspection

  • Darkroom emission filter matched with UVP eLITE excitation filter for fluorescence

  • Roll-out tray inside the darkroom holds the UVP eLITE tray and warming plate

  • Fiber optic guides supplies access from the UVP eLITE MultiSpectral power source for epi illumination inside the darkroom

  • Access port for connecting to an external anesthesia unit (optional)


Warming Plate

Warming plate provides a platform for maintaining a regulated temperature of small animals to prevent hypothermia. The warming plate supplies a uniform temperature setting to calm the animal while taking pictures.


UVP eLITE MultiSpectral Source

  • The UVP eLITE supplies a direct source using fiber optic bundles and a closed optical path to tightly control the output spectrum for consistent, repeatable measurements

  • Adjust the six-position intensity settings as necessary for the desired intensity output

  • External power supply houses the interchangeable excitation filter

  • Fiber optic light source connects to the darkroom for epi illumination


Camera/Lens

  • High resolution, 12 bit color CCD camera

  • 12.5-75mm f1.2 manual zoom lens

  • Permits a field of view (FOV) of 20 x 26cm


VisionWorks Image Acquisition and Analysis Software

VisionWorks analysis software loads on a separate computer for further analysis or documentation. Capabilities include:

  • Quick one-touch outline and identification of tumors

  • Background reduction with excitation/emission sampling (BREES) feature enables automated removable of background

  • Measuring linear dimensions of tumor growths

  • Compositing of multiple fluorescent images

  • Measuring volume of mouse tumors

  • Determining tumor area density

  • Support for 21 CFR Part 11 compliance

  • One-touch automated macros and templates (pre-set or user-defined)

  • Generate extensive reports and export data

  • Multiple user network license available


Partnering with AntiCancer, Inc. for Fluorescent In Vivo Imaging Technology

UVP is partnering with AntiCancer, Inc. for fluorescent protein in vivo small animal imaging technology! UVP's innovative world class iBox system provides researchers with access to extensive techniques and applications technology from AntiCancer, Inc., equipping labs with comprehensive tools for small animal in vivo fluorescent imaging.


SPECIFICATIONS


MultiCam 310C Camera:

  • CCD Bit Depth: 12 bit
    File Bit Depth: 16 bit (analog to digital)

  • Type: Color

  • Grayscale Range: 65,536

  • Pixel Resolution: 1600 x 1200

  • Megapixels: 2.0

  • Captured Image Size (TIF): 3.7mb @ 16-bit


Lens:

  • 12.5-75mm f/1.2 manual zoom

  • Field of View (FOV): 26 x 26 cm

Darkroom:

  • 15" color screen with touch technology

  • Built in computer

  • Light tight chamber

  • Wide access door with viewing window

  • Epi white light

  • Integrated USB ports

  • Emission Filter - GFP (515nm longpass); additional filters available

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Capture Software:

  • Controls via touch screen interface

  • Controls image capture

  • Choose lighting, filters and other functions from the touch screen


Warming Plate:

    Temperature: 33C - 40C, preset to 37C at factory
  • Temperature over limit switch: Prevents unit from going above 41C

  • Accuracy: 1C of readout temperature

  • Variance: Not to exceed 2C over marked area

  • Display: LCD, digital, non illuminating

  • Physical Dimensions (HxWxD): 3.5" x 11.5" x 8" (8.89 x 29.21 x 20.32cm)

UVP eLITE MultiSpectral Source:

  • Fiber optic bundles connect to epi illumination light heads which are mounted in the darkroom

  • Power source housing external to darkroom

  • Single filter positions - 455-495nm filter included

  • GFP (455-495nm) filter included; additional filters available

  • 150 watt light source

  • Manual controls

  • Six-position intensity settings


VisionWorks Software:

  • UVP’s software requires Microsoft Windows 8, Windows 7 or Vista (32-bit or 64-bit).


Options


  • Anesthesia Units:
    Optional anesthesia equipment provides easy-connect, dedicated ports for inlet and outlet isoflurane gas lines. The heat controlled warmer plate maintains a constant temperature for prevention of mouse hypothermia. Multiple-port manifold allows up to five at one time. A pull-out tray in the darkroom enables hassle-free placement of mice in the darkroom chamber. The anesthesia system features patented non-rebreathing technology.
  • Filter sets: Custom and standard excitation and emission filter sets available.

  • Thermal Printer (open new window for printers) for archive quality prints in seconds

  • AntiCancer's fluorescent protein-expressing cancer cell line and AntiCancer's GFP, RFP and CFP mice.

Ordering Info


System Names Camera
iBox Spectra Imaging System
MultiCam 310C


iBox Spectra includes: Darkroom, integrated computer touch screen, capture software, Multiplex 310C Camera/lens, warming plate, manual control UVP eLITE, one matched excitation (455-495nm) and emission (515 long pass) filter set, VisionWorks analysis software.

Intellectual Property


UVP's iBox Spectra Small Animal Imaging System is sold under license from AntiCancer, Inc. for non-commercial use allowing users to optimize their research under the following extensive and broad fluorescent protein imaging patents:

  • USP 6,232,523 , Metastasis models using green florescent protein (GFP) as a marker

  • USP 6,649,159, Whole-body optical imaging of gene expression and uses thereof

  • USP 6,235,968, Metastasis models using green fluorescent protein (GFP) as a marker

  • USP 6,251,384, Metastasis models using green fluorescent protein (GFP) as a marker

  • USP 6,759,038, Metastasis models using green fluorescent protein (GFP) as a marker

  • USP 6,905,831, Real time measurement of cellular responses

  • Australia 749,338, Metastasis models using green fluorescent protein (GFP) as a marker

  • Australia 2001249297, Whole-body optical imaging of gene expression and uses thereof

  • Japan 3,709,343, Metastasis model using green fluorescent protein (GFP) as a marker

  • Japan 4,021,197, Whole-body optical imaging of gene expression and uses thereof

Use of the iBox® Spectra Small Animal Imaging System is limited to the scope of the above listed patents and does not necessarily provide the user with complete freedom to operate as there may be third-party intellectual property rights that apply to a specific application the user may wish to perform. Some of these applications may require additional licensing under patents held by third parties. UVP's sale of the iBox® Spectra Small Animal Imaging System does not convey any right or license under such third party patents and users are advised to consult with their advisors to determine whether licensing of such third-party patents is required for particular applications.



PUBLICATIONS


AntiCancer Application Publications

    Tracking Small Animal Tumor Growth and Treatment

    Imaging cancer dynamics in vivo at the tumor and cellular level with fluorescent proteins, Robert M. Hoffman, Clin Exp Metastasis (2009) 26:345-355 [PDF]

    The multiple uses of fluorescent proteins to visualize cancer in vivo. Nature Reviews Cancer, 2005. Hoffman, R.M. Res. 5, 796-806. [PDF]

    Whole-body imaging of bacterial infection and antibiotic response. Nature Protocols 1(6), 2988-2994, 2007. [PDF]

    Chemotherapy targets the hair-follicle vascular network but not the stem cells. J. Invest. Dermatol. 127, 11-15, 2007 [PDF]

    Photodynamic therapy of rhabdomyosarcoma. Int. J. Oncol. 30, 615-620, 2007 [PDF]

    Whole-body subcellular multicolor imaging. Proc. of SPIE 6449, 64490V1-64490V9, 2007 [PDF]

    Preclinical fluorescent mouse models of pancreatic cancer. Proc. of SPIE 6449, 64490X1-64490X11, 2007 [PDF]

    GFP-expressing vascularization of Gelfoam as a rapid in vivo assay of angiogenesis stimulators and inhibitors. BioTechniques 42, 294-298, 2007. [PDF]

    Noninvasive imaging for evaluation of the systemic delivery of capsid-modified adenovirus in an orthotopic model of advanced lung cancer. Cancer 109, 1213-1214, 2007. [PDF]

    The camptothecin derivative CPT-11 inhibits angiogenesis in a dual-color imageable orthotopic metastatic nude mouse model of human colon cancer. Anticancer Res. 27, 713-718, 2007. [PDF]

    Whole-body subcellular multicolor imaging of tumor-host interaction and drug response in real time. Cancer Res. 67, 5195-5200, 2007. [PDF]

    Dual-color imaging of angiogenesis and its inhibition in bone and soft tissue sarcoma. J. Surg. Res. 140, 165-170, 2007. [PDF]

    Monotherapy with a tumor-targeting mutant of Salmonella typhimurium cures orthotopic metastatic mouse models of human prostate cancer. Proc. Natl. Acad. Sci. USA 104, 10170-10174, 2007. [PDF]

    Mutant PIK3CA-bearing colon cancer cells display increased metastasis in an orthotopic model. Cancer Res. 67, 5851-5858, 2007. [PDF]

    An imageable metastatic treatment model of nasopharyngeal carcinoma. Clin. Cancer Res. 13, 3960-3967, 2007.[PDF]

    A novel alkylating agent, glufosfamide, enhances the activity of gemcitabine in vitro and in vivo. Neoplasia 9, 625-633, 2007. [PDF]

    Real-time imaging of tumor-cell shedding and trafficking in lymphatic channels. Cancer Res. 67, 8223-8228, 2007.[PDF]

    Subcellular imaging of cancer calls in live mice. In: Reporter Genes: A Practical Guide, Voll. 411, pp. 121-130, Methods in Molecular Biology series, Anson, D.S., editor. Humana Press, 2007. [PDF]

    A better fluorescent protein for whole-body imaging. Trends in Biotechnology 26, 1-3, 2008. [PDF]

    Induction of cancer metastasis by cyclophosphamide pretreatment of host mice: an opposite effect of chemotherapy. Cancer Res. 68, 516-520, 2008. [PDF]

    Imaging in mice with fluorescent proteins: from macro to subcellular. Sensors 8, 1157-1173, 2008. [PDF]

    Imaging of cell trafficking and metastasis of pediatric rhabdomyosarcoma. Cell Proliferation 41, 365-374, 2008. [PDF]

    Characterization of HCT116 human colon cancer cells in an orthotopic model. J. Surg. Res. 147, 276-281, 2008. [PDF]

    Biomimetic amplification of nanoparticle homing to tumors. Proc. Natl. Acad. Sci. USA 104, 932-936, 2007. [PDF]



Application Publications


Fluorescence-Guided Surgery of Retroperitoneal-Implanted Human Fibrosarcoma in Nude Mice Delays or Eliminates Tumor Recurrence and Increases Survival Compared to Bright-Light Surgery


Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo


In Vivo Analysis of Bacterial Targeting in Mouse Cancer Models


Development of the Transgenic Cyan Fluorescent Protein (CFP)-Expressing Nude Mouse for "Technicolor" Cancer Imaging


Tracking Small Animal Tumor Growth and Treatment


Targeted therapy of spinal cord glioma with a genetically modified Salmonella typhimurium, Cell Prolif. 2010, 43, 41-48 [PDF]


Imaging cancer dynamics in vivo at the tumor and cellular level with fluorescent proteins, Robert M. Hoffman, Clin Exp Metastasis (2009) 26:345-355 [PDF]


The Cyan Fluorescent Protein (CFP) Transgenic Mouse as a Model for Imaging Pancreatic Exocrine CellsJournal: JOP. J Pancreas (Online) 2009; 10(2):152-156. JOP.J Pancreas (Online) 2009. [PDF]


The multiple uses of fluorescent proteins to visualize cancer in vivo. Nature Reviews Cancer, 2005. Hoffman, R.M. Res. 5, 796-806. [PDF]


Whole-body imaging of bacterial infection and antibiotic response. Nature Protocols 1(6), 2988-2994, 2007. [PDF]


Chemotherapy targets the hair-follicle vascular network but not the stem cells. J. Invest. Dermatol. 127, 11-15, 2007 [PDF]


Photodynamic therapy of rhabdomyosarcoma. Int. J. Oncol. 30, 615-620, 2007 [PDF]


Whole-body subcellular multicolor imaging. Proc. of SPIE 6449, 64490V1-64490V9, 2007 [PDF]


Preclinical fluorescent mouse models of pancreatic cancer. Proc. of SPIE 6449, 64490X1-64490X11, 2007 [PDF]


GFP-expressing vascularization of Gelfoam as a rapid in vivo assay of angiogenesis stimulators and inhibitors. BioTechniques 42, 294-298, 2007. [PDF]


Noninvasive imaging for evaluation of the systemic delivery of capsid-modified adenovirus in an orthotopic model of advanced lung cancer. Cancer 109, 1213-1214, 2007. [PDF]


The camptothecin derivative CPT-11 inhibits angiogenesis in a dual-color imageable orthotopic metastatic nude mouse model of human colon cancer. Anticancer Res. 27, 713-718, 2007. [PDF]


Whole-body subcellular multicolor imaging of tumor-host interaction and drug response in real time. Cancer Res. 67, 5195-5200, 2007. [PDF]


Dual-color imaging of angiogenesis and its inhibition in bone and soft tissue sarcoma. J. Surg. Res. 140, 165-170, 2007. [PDF]


Monotherapy with a tumor-targeting mutant of Salmonella typhimurium cures orthotopic metastatic mouse models of human prostate cancer. Proc. Natl. Acad. Sci. USA 104, 10170-10174, 2007. [PDF]


Mutant PIK3CA-bearing colon cancer cells display increased metastasis in an orthotopic model. Cancer Res. 67, 5851-5858, 2007. [PDF]


An imageable metastatic treatment model of nasopharyngeal carcinoma. Clin. Cancer Res. 13, 3960-3967, 2007.[PDF]


A novel alkylating agent, glufosfamide, enhances the activity of gemcitabine in vitro and in vivo. Neoplasia 9, 625-633, 2007. [PDF]


Real-time imaging of tumor-cell shedding and trafficking in lymphatic channels. Cancer Res. 67, 8223-8228, 2007.[PDF]


Subcellular imaging of cancer calls in live mice. In: Reporter Genes: A Practical Guide, Voll. 411, pp. 121-130, Methods in Molecular Biology series, Anson, D.S., editor. Humana Press, 2007. [PDF]


A better fluorescent protein for whole-body imaging. Trends in Biotechnology 26, 1-3, 2008. [PDF]


Induction of cancer metastasis by cyclophosphamide pretreatment of host mice: an opposite effect of chemotherapy. Cancer Res. 68, 516-520, 2008. [PDF]


Imaging in mice with fluorescent proteins: from macro to subcellular. Sensors 8, 1157-1173, 2008. [PDF]


Imaging of cell trafficking and metastasis of pediatric rhabdomyosarcoma. Cell Proliferation 41, 365-374, 2008. [PDF]


Characterization of HCT116 human colon cancer cells in an orthotopic model. J. Surg. Res. 147, 276-281, 2008. [PDF]


Biomimetic amplification of nanoparticle homing to tumors. Proc. Natl. Acad. Sci. USA 104, 932-936, 2007. [PDF]