Application Notes

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Nucleic Acid Gel Documentation

Safe Gel Imaging with Blue Light Excitation Using GelGreen™ Dye  FP-170

Quantitation of Lanes and Bands using 1D Analysis  FP-160

Next Generation Gel Imaging using GelRed™ and GelGreen™ Dyes with the GelDoc-It® Imaging System  FP-153

DNA Quantitation using Agarose Electrophoresis Densitometry  FP-123

Using the UVP BioDoc-It2 Imaging System for Analysis of Differential Display RT-PCR Gels  FP-119

Products Used: GelDoc-It Imaging System and LabWorks Software

Protein Gel Imaging

Ultra Sensitive Protein Detection and Imaging with Lumitein™ Protein Gel Stain and GelDoc-It Imaging System  FP-154

Quantitative DNA and Protein Analysis with a Highly Uniform UV Illumination Imaging System and Fluorescent Calibration Step Tablet  FP-127

Cytokine Antibody Array Applications with UVP BioImaging Systems   FP-125

Applications of a Highly Uniform UV Illumination Imaging System for Quantitative DNA and Protein Analysis   FP-124

Chemiluminescent Western Blotting

Digital Chemiluminescent Imaging of Protein Blots FP-159

Capture ECLs, Westerns, Chemi Blots with the BioSpectrumAC Imaging System FP-128

Western Blot Imaging with the UVP ChemStudio PLUS (Formerly BioSpectrum)

Optimizing Western Blots for Chemiluminescent Detection with Cooled CCD Camera Imaging Systems  FP-111

Chemiluminescent Immunoblot Detection with Enhanced Luminol HRP Substrates and Digital CCD Camera   FP-117

Enhancement of Chemiluminescent Images   FP-112

Acquiring and Calibrating Chemiluminescent Blots with Visible Light Markers  FP-118

Fluorescent Western Blotting / Multiplexing

Multiplex Fluorescent Western Blot Detection Using the UVP ChemStudio Plus (formerly BioSpectrum) FP-176

Multiplex NIR Imaging of Protein Blots with the UVP ChemStudio PLUS and eLITE™ MultiSpectral Source FP-158

Colony Counting

Colony Measurement Tools Using the ColonyDoc-It™ Imaging Station FP-164

Counting GFP Inhibition Zones with the ColonyDoc-It Imaging Station FP-148

Multi-Color Bacterial Counting with the ColonyDoc-It Imaging Station FP-149

Measuring Inhibition Zones with the ColonyDoc-It Imaging Station FP-143

Radial Diffusion Assay and Analysis for Proteases and Other Hydrolytic Enzymes Using the ColonyDoc-It Imaging Station FP-147

Colony Counting GFP Bacteria with the ColonyDoc-It Imaging Station FP-142


Imaging of Fluorescent Microspheres Using the iBox® Explorer™ Imaging Microscope  FP-175

2D Gel & Blot Imaging

2D Imaging with the UVP ChemStudio PLUS (formerly Biospectrum)  FP-131 2D

Small Animal In Vivo Imaging

Infection of Soybean Plant Roots With GFP-Expressing Phytophthora Sojae, Imaged Using the iBox Explorer2 Imaging Microscope  FP-180

In Vivo Fluorescence Imaging of Angiogenic Vessels in Liver Cancer Animal Model Using the iBox Explorer2 Imaging Microscope  FP-181

Imaging of Tumor Growth and Metastasis in a Pancreatic Cancer Mouse Model  FP-186

In Vivo Imaging of a Cy5.5 Labeled Amyloid-β, an Alzheimer's Disease Associated Protein  FP-185

Low Light Imaging of Reactive Oxidative Species within Wound Sites Using Luminol  FP-179

Biodistribution Monitoring Using the iBox Explorer2 Imaging Microscope  FP-178

Imaging of Near Infrared (NIR) Dyes In Vivo  FP-173

Monitoring Tumor Progression Using the iBox® Scientia™ Imaging System  FP-172

Intravital Imaging of a Mouse Ventral Skin-Flap  FP-171

Detecting a Mouse Pancreatic Cancer Cell Line Using the iBox® Explorer™ Imaging Microscope  FP-169

Real-Time Imaging of Nuclear-Cytoplasmic Dynamics in UV Light Killing of Cancer Cells Expressing Fluorescent Proteins  FP-156

Color Coded Fluorescence Imaging in Mice Using the iBox Small Animal Imaging System  FP-155

Measuring Molecular Mobility with Macro Fluorescence Imaging  FP-151

The Advantages of Fluorescent Proteins Over Luciferase for In Vivo Imaging  FP-129

Salmonella Typhimurium Treatment of RFP Tagged U87 Tumors in Nude Mice (AntiCancer, Inc.)  FP-130

Plant Imaging

Use of Red Firefly Luciferase to Monitor Infiltration of Nicotiana benthamiana for In Planta Bioluminescence FP-184

Infection of Soybean Plant Roots With GFP-Expressing Phytophthora Sojae  FP-180

Genetic Mapping of Newly Identified Mutations Using the Arabidopsis Genome and CAPs Markers  FP-120

General Imaging Topics

Using Flat Field Correction to Compensate for Shadowing Effect When Imaging Microtiter Plates FP-122

Understanding and Detection of Green Fluorescent Proteins (GFP) and Their Variants  FP-121

Understanding CCD Cameras  FP-114

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Analytik Jena US's products can be used in many different applications. Application Notes describe specific uses for some of Analytik Jena US's products. For applications not shown here, contact Analytik Jena US's offices.