UVP iBox® Explorer2

UVP iBox® Explorer²™ Imaging Microscope

Macro to Micro Fluorescent In Vivo Small Animal Imaging

Now researchers can take detection of fluorescent markers in small animals to a new level with the iBox Explorer² Imaging Microscope! The iBox Explorer² is unique in its ability to view macro to micro in the whole animal to individual cell, subcutaneously and within the body cavity of mice. The upright optics provide an ultra long working distance and high numerical aperture (NA) for detailed fluorescent in vivo imaging.

The UVP iBox Explorer² easily detects GFP/RFP and other fluorescent markers in small animals. The capabilities of this in vivo small animal imaging system enables research studies in whole animal down to individual cells.

Applications

 

• Tumor shedding

• Tumor/host margins and interactions

• Hematogenous trafficking

• Tumor angiogenesis

• Tumor micro environment

• Intralymphatic trafficking

• Micro/macro metastases

• Primary tumor growth

• Biodistribution

Main Features


  • Magnification ranges of 0.17x - 16.5x enables easy transition from the macroscopic to the microscope scale

  • iBox Explorer² enables researchers to visualize micro injection of cancer cells in vivo

  • Ability to image organs and cells subcutaneously and within the body cavity of living mice

  • Optical configurations are parcentered and parfocal, allowing seamless imaging through the magnification ranges

  • Leading-edge high frame rate cooled color camera enables quick detection, image capture and high throughput

  • Easy to use software automates research with templates for reproducible, consistent results

  • Bright illumination of samples produces an intense fluorescent signal and fast exposure times

  • Unlimited selection of filters enables users to image in the fluorescent, visible and NIR ranges for multiple applications (RFP/GFP included, others optional)

  • Generate accurate data with VisionWorks® Software



UVP iBox® Explorer² Imaging Microscope is sold under license from AntiCancer, Inc. allowing scientists to optimize their research using an extensive array of patented fluorescent protein imaging applications technologies.



Product Info


Focusing on Fluorescent Cancer Cell Detection

In research performed, HT1080 fluorescent cancer cells were injected into the epigastrica cranialis. Immediately after the injection, the fluorescence indicates the cells escaped around the injection sites. In the zoomed in views, a cluster of cancer cells can be observed in the blood stream.



System Capabilities
  • Capture images with a superior cooled CCD camera and optics that are optimized for VIS - NIR imaging

  • View the whole animal to the single cell level via the motorized optics

  • Seamlessly navigate the image through all magnification ranges from 0.17x to 16.5x

  • Control eLITE excitation filters and light intensity via the software interface

  • Generate continuous excitation spectrum with the configurable filters for fluorescence microscopy

  • Slide-out warming plate maintains uniform temperature

  • Create optimum imaging conditions with the light tight darkroom

  • See quick sample inspection with unique viewing window

  • Simply adjust the position of the motorized X, Y, Z stage with the joystick



UVP eLITE Xe MultiSpectral Light Source
  • Easy installation of the UVP eLITE Xe external light source through the darkroom access ports

  • Epi fiber optic Xenon light source supplies uniform and directed lighting to the animal

  • Matched excitation/emission green fluorescent protein (GFP), red fluorescent protein (RFP) sets included; custom filter sets available

  • Eight excitation filter positions accommodate a variety of filters for specific applications

  • Six intensity settings from 0 to 100% relative intensity regulate the amount of illumination supplied through the fiber optic cable



Application eLITE Excitation
Wavelength
Microscope Emission
Wavelength
GFP 455-495nm 503-523nm
RFP 502-547nm 580-630nm
General   515 long pass
Neutral Density   400-700nm


Emission filters are placed in the darkroom filter tray. The neutral density filter passes emission wavelengths from 400-700nm. This filter is used for white light or non-fluorescent imaging. The long pass filter blocks blue light excitation and passes fluorescence.


Additional standard and custom filter sets are available to cover a wide spectra range of fluorescent proteins. For an extensive list filters available, go to Filters.


VisionWorks® Image Acquisition and Analysis Software

VisionWorks Image Acquisition and Analysis Software can be configured with the system or ordered separately. Check with your local representative for details. This sophisticated package provides comprehensive tools for image capture, enhancement and analysis. Capabilities include:

  • Image acquisition functions for simple image capture and expanded integration options

  • Estimate tumor volume

  • Measure linear dimensions of tumors

  • Apply numerous image enhancements to images

  • Comprehensive tools for advanced analysis including area density

  • Support for 21 CFR Part 11 compliance

  • One-touch automated macros

  • User defined templates, profiles and preferences

  • Generate extensive reports and export data

  • Multiple user network licenses available

  • Enhancement and analysis tools are included that allow researchers to personalize their experiments for any special purpose. Tools offered: background subtraction, noise reduction, inversion, compositing, pseudocolor, and more.

  • Each user can designate a password to store and protect data in a busy research environment.



Specifications


Darkroom Specifications

Emission Filters Four-position interchangeable rack:
  • 515nm Longpass, GFP, RFP, Neutral Density

  • Additional wavelength filters available

Controls Automated, software controlled
Stage Precision motorized X, Y, Z
Travel X=100mm, Y=100mm, Z=100mm
Resolution X=10μm, Y=10μm, Z=1μm
Position/Focus Controlled by joystick/software
Warming Plate 37°C, user adjustable
Darkroom Dimensions 17.5"W x 19.5"D x 41"H (44.5 x 49.5 x 104cm)
Light Sources 150 watt Xenon
Fiber Optic Coaxial and epi illumination with light heads mounted to the darkroom
Excitation Filters Eight position wheel includes filters - GFP, RFP (others available)
Controls Via software
eLITE Dimensions 12.5 x 13.5 x 10 in. (31.8 x 34.3 x 25.4cm)
Camera OptiChemi 615
  • Monochrome

  • 16 bit

  • 2184 x 1472 pixel Resolution

  • 3.2, extendable to 9.6 Megapixels

  • Cooling: -60°C from ambient

  • Peak QE: 88% (quantum efficiency)

Software

Interface

  • Camera, objectives, darkroom, eLITE

Tools

  • Macros and templates

Analysis

  • Extensive functions including area density measurements, volume measurements and tumor sizing

Documentation

  • Create reports and export data

Compliance

  • Supports 21 CFR Part 11

UVP’s software requires Microsoft Windows 7,8, or 10 (32-bit or 64-bit).



Magnification and Imaging Area

Optical Magnification:
0.17x 0.25x 0.5x 1.66x 2.5x 4.5x 7.5x 8.8x 16.5x
FOV Observation Range:
90x90mm 60x60mm 30x30mm 9x9mm 6x6mm 3.3x3.3mm 2x2mm 1.7x1.7mm 0.9x0.9mm
eLITE XE MultiSpectral Light Source

Options


Anesthesia Unit

  • Optional anesthesia unit for small animals produces a state of immobilization that allows acquisition of repeatable and reliable data Anesthesia Station



Order Information


  • iBox Explorer² 615 Imaging Microscope



Application Materials


Application Notes, Posters and Reference Articles

Human RAGE expressed in endothelial cells exacerbates blood-brain barrier dysfunction S. V. REGE1, E. HATCH2, M. WANG1, N. CHUQUI1, Z. ZHAO1, A. P. SAGARE1, B. V. ZLOKOVIC1, 2 1Physiology and Biophysics, USC, Los Angeles, CA; 2Ctr. for Neurodegenerative and Vascular Brain Disorders, Univ. of Rochester, Rochester, NY Poster (2.6MB)


CD137 signaling enhances tight junction resistance in intestinal epithelial cells, Veronica Gusti, Kaila M. Bennett , David D. Lo, Physiological Reports Published 5 August 2014 Vol. 2 no. e12090DOI: 10.14814/phy2.12090 read the article


Imaging of Fluorescent Microspheres Using the iBox® Explorer™ Imaging Microscope FP-175


In Vivo Imaging of a Cy5.5 Labeled Amyloid-β, an Alzheimer's Disease Associated Protein FP-185


Imaging of Fluorescent Metastatic Mouse Models of Pancreatic Cancer Using the iBox Explorer2 Imaging Microscope FP-182


Intravital Imaging of a Mouse Ventral Skin-Flap FP-171


Detecting a Mouse Pancreatic Cancer Cell Line Using the iBox® Explorer™ Imaging Microscope FP-169


In Vivo Fluorescence Imaging of Angiogenic Vessels in Liver Cancer Animal Model Using the iBox Explorer² Imaging Microscope FP-181


Infection of Soybean Plant Roots With GFP-Expressing Phytophthora Sojae, Imaged Using the iBox Explorer² Imaging Microscope FP-180


Biodistribution Monitoring Using the iBox Explorer² Imaging Microscope; Quantitative and Qualitative Measurement of Near Infrared Dye Distribution in a Nude Mouse FP-178


Video/Webinar

In Vivo Imaging - Individual Cell and Whole Organ Analysis

Watch the webinar Webinar: Fluorescence In Vivo Imaging