UVP IBOX® SCIENTIA

UVP iBox® Scientia™ Small Animal Imaging System

UVP iBox Scientia Small Animal Imaging System is designed to automate your in vivo small animal imaging research. System enables non-invasive detection of fluorescent and bioluminescent reporters in mice. The one-touch, pre-set or user-defined controls are easy to use for accurate, repeatable imaging.

Applications

 

Fluorescent and Bioluminescent Applications for the UVP iBox Scientia for pre-clinical research include:

• Tumor studies

•Cancer research

•Heart disease

•Gene therapy

System NamesCamera
UVP iBox Scientia 615 Imaging SystemBioCam 900
UVP iBox Scientia 900 Imaging SystemOptiChemi 615

Main Features


  • Selection of high resolution, high sensitivity CCD cameras for in vivo imaging
  • Motorized optics for setting the aperture, zoom and focus
  • Light tight darkroom creates optimum imaging conditions
  • Unique viewing window enables quick sample inspection
  • Matched emission/excitation filter sets specifically for small animal imaging
  • Automated control of the five-position emission filter array to allow addition of emissions for visible, IR and UV to meet all spectral requirements
  • Warming plate
  • Roll-out tray provides easy access for placement of mice on the warming plate
  • Automated templates for repeated experiments
  • VisionWorks® Software (configured with system or optional) for analysis and image compositing


Product Info


Darkroom

  • Light tight darkroom creates optimum imaging conditions

  • Unique viewing window is UV blocking for quick sample inspection

  • Built-in overhead white light and UV are easily selected from the software interface

  • Automated control of the five-position emission filter array

  • Power lift is adjustable to any setting in a ten inch travel range (manual lift also available); Automated calibrating home position synchronizes the power lift at start-up

  • Dual fiber optic ports supply access from the UVP eLITE MultiSpectral power source to the epi or eLITE transilluminator lights
  • Access port for connecting to an external anesthesia unit (not included)

Warming Plate

Warming Plate provides a platform for maintaining a regulated temperature of small animals to prevent hypothermia. The stable temperature enables consistent day-to- day results. Plate supplies a uniform temperature setting to calm the animal while taking pictures.

UVP eLITE MultiSpectral Source

  • External power supply houses the eight position filter wheel

  • Automated design controls the filter wheel and six intensity positions

  • Fiber optic light source connects through the darkroom port for epi or transillumination

  • Epi illumination is built into the darkroom while the eLITE transilluminator is an optional unit

  • Matched excitation/emission green fluorescent protein (GFP) and red fluorescent protein (RFP) excitation filter are included; other filter sets available

  • The eLITE supplies a direct source using fiber optic bundles and a closed optical path to tightly control the output spectrum for consistent, repeatable measurements


Camera/Lens

Choose from two camera models: BioCam 900 or OptiChemi 615. Link to camera specifications for more information. Cameras are selected for their high sensitivity for in vivo imaging applications. The configurations supply real time, live preview of images.


Software Interface

  • Software automates your research for easy, yet accurate and repeatable results

  • Controls the eLITE, darkroom’s epi and transillumination UV and white light, emission filter selection and system power

  • Allows selection of user-defined or default settings

  • Select identify specific filters

  • Click on the epi and transillumination light sources

  • Set templates and user preferences for recurring experiments

VisionWorks Image Acquisition and Analysis Software

VisionWorks Image Acquisition and Analysis Software can be configured with the system or ordered separately. Check with your local representative for details. This sophisticated package provides comprehensive tools for image capture and analysis of gels, plates and membranes as well as colony counting. Capabilities include:

  • Image acquisition functions for simple image capture and expanded integration options

  • Apply numerous image enhancements to images

  • Comprehensive tools for advanced 1D lane densitometry and analysis including area density, molecular weights and colony counting

  • Support for 21 CFR Part 11 compliance

  • One-touch automated macros for repeated actions

  • Define user profiles and preferences

  • Generate extensive reports and export data

  • Multiple user network license available

  • Enhancement and analysis tools are included that allow researchers to personalize their experiments for any special purpose. Tools offered: background subtraction, noise reduction, inversion, compositing, pseudocolor, and more.

  • Each user can designate a password to store and protect data in a busy research environment.



Specifications


Camera Options BioCam 900
  • 1024 x 1024
  • 1.0 Megapixels
  • >90% (Visible)
  • 42.5mm f/0.95 or 25mm f/0.95
  • USB 2.0 PC Connection
OptiChemi 615
  • 2184 x 1472
  • 3.2 Megapixels, extend to 9.6
  • 25mm f/0.95 or 50mm f/1.2
  • USB 2.0 PC Connection
Darkroom
  • Light tight
  • Wide access door with UV safety shutoff
  • Gel viewing window
  • Sliding tray for optional transilluminator
  • Epi white and UV illumination (365nn and 480nm)
Stage
  • Temperature: 33°C - 40°C, preset to 37°C at factory
  • Temperature over limit switch: Prevents unit from going above 41°C
  • Accuracy: 1°C of readout temperature
  • Variance: Not to exceed 2°C over marked area
  • Display: LCD, digital, non-illuminating
  • Physical Dimensions (HxWxD): 3.5” x 11.5” x 8” (8.89 x 29.21 x 20.32cm)
Emission Filters-five positions
  • green Fluorescent Protein (GFP)
  • Red Fluorescent Protein (RFP)
UVP eLITE MultiSpectral Light Source
  • Power source housing external to darkroom
  • 150 watt light source
  • Fiber optic bundles connect to either epi or transillumination light sources
  • Eight excitation filter positions - GFP and RFP included (additional filters available)
  • Manual or software controlled
Software
  • Interface for automated controls
  • VisionWorks Acquisition and Analysis Software (system may be configured with software or optional, check with your AJ representative)
  • AJ’s software requires Microsoft Windows 7, 8, or 10 (32-bit or 64-bit).operating systems.


Options


Anesthesia Unit

  • Anesthesia Units:Optional anesthesia equipment provides easy-connect, dedicated ports for inlet and outlet isoflurane gas lines. The heat controlled warmer plate maintains a constant temperature for prevention of mouse hypothermia. Multiple-port manifold allows up to five at one time. A pull-out tray in the darkroom enables hassle-free placement of mice in the darkroom chamber. The anesthesia system features patented rebreathing technology.

  • Custom and standard excitation and emission filter sets
  • Thermal Printer: For archive quality prints in seconds
  • LED White Light Tray
  • UVP Benchtop and UVP FirstLight UV transilluminators
  • Computer and monitor
  • AntiCancer's fluorescent protein-expressing cancer cell line and AntiCancer's GFP, RFP and CFP mice.


Ordering Information


  • UVP iBox Scientia 615 Imaging System

  • UVP iBox Scientia 900 Imaging System



Intellectual Property


Analytik Jena's UVP iBox® Scientia Small Animal Imaging System is sold under license from AntiCancer, Inc. for non-commercial use allowing users to optimize their research under the following extensive and broad fluorescent protein imaging patents:

  • USP 6,232,523 , Metastasis models using green florescent protein (GFP) as a marker
  • USP 6,649,159, Whole-body optical imaging of gene expression and uses thereof
  • USP 6,235,968, Metastasis models using green fluorescent protein (GFP) as a marker
  • USP 6,251,384, Metastasis models using green fluorescent protein (GFP) as a marker
  • USP 6,759,038, Metastasis models using green fluorescent protein (GFP) as a marker
  • USP 6,905,831, Real time measurement of cellular responses
  • Australia 749,338, Metastasis models using green fluorescent protein (GFP) as a marker
  • Australia 2001249297, Whole-body optical imaging of gene expression and uses thereof
  • Japan 3,709,343, Metastasis model using green fluorescent protein (GFP) as a marker
  • Japan 4,021,197, Whole-body optical imaging of gene expression and uses thereof

Use of the iBox® Scientia Small Animal Imaging System is limited to the scope of the above listed patents and does not necessarily provide the user with complete freedom to operate as there may be third-party intellectual property rights that apply to a specific application the user may wish to perform. Some of these applications may require additional licensing under patents held by third parties. AJ's sale of the iBox® Scientia Small Animal Imaging System does not convey any right or license under such third party patents and users are advised to consult with their advisors to determine whether licensing of such third-party patents is required for particular applications


Application Publications


Fluorescence-Guided Surgery of Retroperitoneal-Implanted Human Fibrosarcoma in Nude Mice Delays or Eliminates Tumor Recurrence and Increases Survival Compared to Bright-Light Surgery


Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo


In Vivo Analysis of Bacterial Targeting in Mouse Cancer Models


Development of the Transgenic Cyan Fluorescent Protein (CFP)-Expressing Nude Mouse for "Technicolor" Cancer Imaging


Tracking Small Animal Tumor Growth and Treatment


Targeted therapy of spinal cord glioma with a genetically modified Salmonella typhimurium, Cell Prolif. 2010, 43, 41-48 [PDF]


Imaging cancer dynamics in vivo at the tumor and cellular level with fluorescent proteins, Robert M. Hoffman, Clin Exp Metastasis (2009) 26:345-355 [PDF]


The Cyan Fluorescent Protein (CFP) Transgenic Mouse as a Model for Imaging Pancreatic Exocrine CellsJournal: JOP. J Pancreas (Online) 2009; 10(2):152-156. JOP.J Pancreas (Online) 2009. [PDF]


The multiple uses of fluorescent proteins to visualize cancer in vivo. Nature Reviews Cancer, 2005. Hoffman, R.M. Res. 5, 796-806. [PDF]


Whole-body imaging of bacterial infection and antibiotic response. Nature Protocols 1(6), 2988-2994, 2007. [PDF]


Chemotherapy targets the hair-follicle vascular network but not the stem cells. J. Invest. Dermatol. 127, 11-15, 2007 [PDF]


Photodynamic therapy of rhabdomyosarcoma. Int. J. Oncol. 30, 615-620, 2007 [PDF]


Whole-body subcellular multicolor imaging. Proc. of SPIE 6449, 64490V1-64490V9, 2007 [PDF]


Preclinical fluorescent mouse models of pancreatic cancer. Proc. of SPIE 6449, 64490X1-64490X11, 2007 [PDF]


GFP-expressing vascularization of Gelfoam as a rapid in vivo assay of angiogenesis stimulators and inhibitors. BioTechniques 42, 294-298, 2007. [PDF]


Noninvasive imaging for evaluation of the systemic delivery of capsid-modified adenovirus in an orthotopic model of advanced lung cancer. Cancer 109, 1213-1214, 2007. [PDF]


The camptothecin derivative CPT-11 inhibits angiogenesis in a dual-color imageable orthotopic metastatic nude mouse model of human colon cancer. Anticancer Res. 27, 713-718, 2007. [PDF]


Whole-body subcellular multicolor imaging of tumor-host interaction and drug response in real time. Cancer Res. 67, 5195-5200, 2007. [PDF]


Dual-color imaging of angiogenesis and its inhibition in bone and soft tissue sarcoma. J. Surg. Res. 140, 165-170, 2007. [PDF]


Monotherapy with a tumor-targeting mutant of Salmonella typhimurium cures orthotopic metastatic mouse models of human prostate cancer. Proc. Natl. Acad. Sci. USA 104, 10170-10174, 2007. [PDF]


Mutant PIK3CA-bearing colon cancer cells display increased metastasis in an orthotopic model. Cancer Res. 67, 5851-5858, 2007. [PDF]


An imageable metastatic treatment model of nasopharyngeal carcinoma. Clin. Cancer Res. 13, 3960-3967, 2007.[PDF]


A novel alkylating agent, glufosfamide, enhances the activity of gemcitabine in vitro and in vivo. Neoplasia 9, 625-633, 2007. [PDF]


Real-time imaging of tumor-cell shedding and trafficking in lymphatic channels. Cancer Res. 67, 8223-8228, 2007.[PDF]


Subcellular imaging of cancer calls in live mice. In: Reporter Genes: A Practical Guide, Voll. 411, pp. 121-130, Methods in Molecular Biology series, Anson, D.S., editor. Humana Press, 2007. [PDF]


A better fluorescent protein for whole-body imaging. Trends in Biotechnology 26, 1-3, 2008. [PDF]


Induction of cancer metastasis by cyclophosphamide pretreatment of host mice: an opposite effect of chemotherapy. Cancer Res. 68, 516-520, 2008. [PDF]


Imaging in mice with fluorescent proteins: from macro to subcellular. Sensors 8, 1157-1173, 2008. [PDF]


Imaging of cell trafficking and metastasis of pediatric rhabdomyosarcoma. Cell Proliferation 41, 365-374, 2008. [PDF]


Characterization of HCT116 human colon cancer cells in an orthotopic model. J. Surg. Res. 147, 276-281, 2008. [PDF]


Biomimetic amplification of nanoparticle homing to tumors. Proc. Natl. Acad. Sci. USA 104, 932-936, 2007. [PDF]